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KMID : 0613819990090020146
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Journal of Life Science
1999 Volume.9 No. 2 p.146 ~ p.152
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Effect of Substituted Residue 24 on Folding of Trytophan Synthase $\alpha$ Subunit
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Abstract
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In order to elucidate a role of residue 24 in the folding of tryptophan synthase $\alpha$ subunit, mutant proteins in which Thr 24 was replaced Met, Ala, Ser, Leu or Lys were overexpressed in E. coli, and the extents of accumulated proteins as soluble or aggregated forms were examined. The mutant proteins with Met or Leu at residue 24 were predominatly accumulated as expressed as aggregated forms as well. This result suggests that residue 24 of tryptophan synthase $\alpha$ subuunit may be implicated in the folding of this protein,
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